Journal: International immunopharmacology

Article Title: Sophora subprostrate polysaccharide targets LncRNA MSTRG.5823.1 to suppress PCV2-mediated immunosuppression via TNF/NF-κB signaling.

doi: 10.1016/j.intimp.2024.112701

Figure Lengend Snippet: Fig. 4. Validation of differentially expressed mRNAs, lncRNAs, and miRNAs (n ¼ 3). Notes: (A) mRNA expression levels of Ccl4, Clec4e, CXCL2, Acod1, Bcl2a1d, Ccl3, Dusp2, Dusp5, Glrx, IL27, Itga5, Nfkbia, Nfkbid, Nfkbiz, Nlrp3, Pdgfb, Tnf and Tnfrsf1b. (B) lncRNA expression levels of ENSMUST00000174829, ENSMUST0000034789, ENSMUST0000074287, ENSMUST0000022589, MSTRG.12908.1 and MSTRG.5823.1. (C) miRNA expression levels of miR-21-z, miR-17-x, miR-212-x, miR-217-x, and miR-342-y.

Article Snippet: The primary antibody against rabbit CXCL2 (pb0900, 1:500 dilution) was obtained from BOSTER (California, USA).

Techniques: Biomarker Discovery, Expressing

Journal: International immunopharmacology

Article Title: Sophora subprostrate polysaccharide targets LncRNA MSTRG.5823.1 to suppress PCV2-mediated immunosuppression via TNF/NF-κB signaling.

doi: 10.1016/j.intimp.2024.112701

Figure Lengend Snippet: Fig. 5. CXCL2-miR-217-X-MSTRG.5823.1 network construction. Notes: (A) Co differentially expressed mRNA, miRNA and lncRNA in the ctrl vs PCV2 com- parison group and PCV2 vs PCV2 + SSP comparison group shown by Venn diagram. (B) KEGG pathway analysis of 78 differentially co-expressed mRNAs (C) GO pathway analysis of 78 differentially co-expressed mRNAs. (D) Subcellular localization of lncRNA MSTRG.5823.1, with MSTRG.5823.1 probe in red and DAPI nuclear staining in blue, and no overlap between red and blue, indicating that MSTRG.5823.1 is located in the cytoplasm. (E) Prediction of targeting relationship between MSTRG.5823.1-miR-217-x- CXCL2. (F) In the ceRNA network, red represents up-regulation, green represents down-regulation, circles represent mRNA, triangles represent miRNAs, diamonds represent lncRNAs, and the solid line indicates the targeting relationship. (G) Targeted binding relationship between CXCL2 and miR- 217-x (n = 3). (H) Target binding relationship between MSTRG.5823.1 and miR-217-x (n = 3). Error bars are represented as means ± SD. *p value < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: The primary antibody against rabbit CXCL2 (pb0900, 1:500 dilution) was obtained from BOSTER (California, USA).

Techniques: Comparison, Staining, Binding Assay

Journal: International immunopharmacology

Article Title: Sophora subprostrate polysaccharide targets LncRNA MSTRG.5823.1 to suppress PCV2-mediated immunosuppression via TNF/NF-κB signaling.

doi: 10.1016/j.intimp.2024.112701

Figure Lengend Snippet: Fig. 6. Effect of miR-217-x in PCV2-infected RAW264.7 cells (n ¼ 3). Notes: (A) Effect of overexpression miR-217-x on the expression levels of miR-217-x, MSTRG.5823.1, Rel and TNF-α in PCV2 infected RAW264.7 cells. (B) Effect of overexpression miR-217-x on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in PCV2 infection group. (C-E) Effects of overexpression miR-217-x on the expression levels of CXCl2, TNF-α, IKB, p-IKB, IKKβ, p-IKKβ, p65 and p-p65 proteins in PCV2 infected RAW264.7 cells. (F) Effect of knockdown miR-217-x on the expression levels of miR-217-x, MSTRG.5823.1, Rel and TNF-α. (G) Effect of knockdown miR-217-x on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in PCV2 infected RAW264.7 cells. (H-J) Effect of knockdown miR-217-x on the expression levels of CXCL2, TNF-α, IKB, p-IKB, IKKβ, p-IKKβ, p65 and p-p65 proteins in PCV2 infected RAW264.7 cells. Error bars are represented as means ± SD. Shoulder labels with the same alphabet indicate non-significant differences (p > 0.05), while labels with different alphabets indicate significant differences (p < 0.05).

Article Snippet: The primary antibody against rabbit CXCL2 (pb0900, 1:500 dilution) was obtained from BOSTER (California, USA).

Techniques: Infection, Over Expression, Expressing, Knockdown

Journal: International immunopharmacology

Article Title: Sophora subprostrate polysaccharide targets LncRNA MSTRG.5823.1 to suppress PCV2-mediated immunosuppression via TNF/NF-κB signaling.

doi: 10.1016/j.intimp.2024.112701

Figure Lengend Snippet: Fig. 7. The function of miR-217-x in SSP-treated PCV2-infected RAW264.7 cells (n ¼ 3). Notes: (A) Effect of overexpression miR-217-x on the expression levels of miR-217-x, MSTRG.5823.1, Rel and TNF-α in SSP treatment group. (B) Effect of overexpression miR-217-x on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in SSP-treated −RAW264.7 cells infected with PCV2. (C-E) Effect of overexpression miR-217-x on the expression levels of CXCL2, TNF-α, IKB, p-IKB, IKKβ, p- IKKβ, p65and p-p65 proteins in SSP treatment group. (F) Effect of knockdown miR-217-x on the expression levels of miR-217-x, MSTRG.5823.1, Rel and TNF-α in SSP-treated RAW264.7 cells infected with PCV2. (G) Effect of knockdown miR-217-x on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in SSP treatment group. (H-J) Effect of knockdown miR-217-x on the protein expression levels of CXCL2, TNF-α, IKB, p-IKB, IKKβ, p-IKKβ, p65and p-p65 in SSP treatment group. Error bars are represented as means ± SD Shoulder labels with the same alphabet indicate non-significant differences (p > 0.05), while labels with different alphabets indicate significant differences (p < 0.05).

Article Snippet: The primary antibody against rabbit CXCL2 (pb0900, 1:500 dilution) was obtained from BOSTER (California, USA).

Techniques: Infection, Over Expression, Expressing, Knockdown

Journal: International immunopharmacology

Article Title: Sophora subprostrate polysaccharide targets LncRNA MSTRG.5823.1 to suppress PCV2-mediated immunosuppression via TNF/NF-κB signaling.

doi: 10.1016/j.intimp.2024.112701

Figure Lengend Snippet: Fig. 8. The function of lncRNA-MSTRG.5823.1 in PCV2-infected RAW264.7 cells (n ¼ 3). Notes: (A) Effect of overexpression of MSTRG.5823.1 on the expression levels of miR-217-x, MSTRG.5823.1, Rel and TNF-α in PCV2-infected RAW264.7 cells. (B) Effect of overexpression of MSTRG.5823.1 on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in PCV2-infected RAW264.7 cells. (C-E) Effect of overexpression of MSTRG.5823.1 on the protein expression levels of CXCL2, TNF-α, IKB, p-IKB, IKKβ, p-IKKβ, p65and p-p65 in PCV2-infected RAW264.7 cells. (F) Effect of knockdown MSTRG.5823.1 on the expression levels of miR- 217-x, MSTRG.5823.1, Rel and TNF-α. (G) Effect of knockdown MSTRG.5823.1 on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in PCV2-infected RAW264.7 cells. (H-J) Effect of knockdown MSTRG.5823.1 on the protein expression levels of CXCL2, TNF-α, IKB, p-IKB, IKKβ, p-IKKβ, p65 and p-p65 in PCV2- infected RAW264.7 cells. Error bars are represented as means ± SD. Shoulder labels with the same alphabet indicate non-significant differences (p > 0.05), while labels with different alphabets indicate significant differences (p < 0.05).

Article Snippet: The primary antibody against rabbit CXCL2 (pb0900, 1:500 dilution) was obtained from BOSTER (California, USA).

Techniques: Infection, Over Expression, Expressing, Knockdown

Journal: International immunopharmacology

Article Title: Sophora subprostrate polysaccharide targets LncRNA MSTRG.5823.1 to suppress PCV2-mediated immunosuppression via TNF/NF-κB signaling.

doi: 10.1016/j.intimp.2024.112701

Figure Lengend Snippet: Fig. 9. The function of lncRNA-MSTRG.5823.1 in SSP-treated PCV2-infected RAW264.7 cells (n ¼ 3). Notes: (A) Effect of overexpression of MSTRG.5823.1 on the expression levels of miR-217-x, MSTRG.5823.1, Rel, TNF-α in SSP treatment group. (B) Effect of overexpression of MSTRG.5823.1 on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in SSP treatment group. (C-E) Effect of overexpression of MSTRG.5823.1 on the protein expression levels of CXCL2, TNF-α, IKB, p- IKB, IKKβ, p-IKKβ, p65 and p-p65 in SSP treatment group. (F) Effect of knockdown MSTRG.5823.1 on the expression levels of miR-217-x, MSTRG.5823.1, Rel and TNF-α in SSP treatment group. (G) Effect of knockdown MSTRG.5823.1 on the expression levels of CXCL2, CXCL10, IL-1β mRNA in SSP treatment group. (H-J) Effect of knockdown MSTRG.5823.1 on the expression levels of CXCL2, TNF-α, IKB, p-IKB, IKKβ, p-IKKβ, p65 and p-p65 proteins in SSP treatment group. Error bars are represented as means ± SD. Shoulder labels with the same alphabet indicate non-significant differences (p > 0.05), and shoulder labels with different alphabets indicate significant differences (p < 0.05).

Article Snippet: The primary antibody against rabbit CXCL2 (pb0900, 1:500 dilution) was obtained from BOSTER (California, USA).

Techniques: Infection, Over Expression, Expressing, Knockdown

Journal: International immunopharmacology

Article Title: Sophora subprostrate polysaccharide targets LncRNA MSTRG.5823.1 to suppress PCV2-mediated immunosuppression via TNF/NF-κB signaling.

doi: 10.1016/j.intimp.2024.112701

Figure Lengend Snippet: Fig. 10. Effect of the MSTRG.5823.1/miR-217-x/CXCL2 axis on immunosuppression in PCV2-infected RAW264.7 cells (n ¼ 3). Notes: (A) Effect of over- expression of miR-217-x and MSTRG.5823.1 on the expression levels of miR-217-x, MSTRG.5823.1, Rel and TNF-α in PCV2 infection group. (B) Effect of over- expression of miR-217-x and MSTRG.5823.1 on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in in PCV2 infection group. (C-E) Effect of overexpression of miR-217-x and MSTRG.5823.1 on CXCL2, TNF-α, IKB, p-IKB, IKKβ, p-IKKβ, p65 and p-p65 protein expression levels in in PCV2 infection group. (F) Effect of knockdown miR-217-x and MSTRG.5823.1 on the expression levels of miR-217-x, MSTRG.5823.1, Rel and TNF-α. (G) Effect of knockdown miR-217-x and MSTRG.5823.1 on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in PCV2-infected RAW264.7 cells. (H-J) Effect of knockdown miR-217-x and MSTRG.5823.1 on the protein expression levels of CXCL2, TNF-α, IKB, p-IKB, IKKβ, p-IKKβ, p65 and p-p65 in PCV2-infected RAW264.7 cells. Error bars are rep- resented as means ± SD. Shoulder labels with the same alphabet indicate non-significant differences (p > 0.05), while labels with different alphabets indicate significant differences (p < 0.05).

Article Snippet: The primary antibody against rabbit CXCL2 (pb0900, 1:500 dilution) was obtained from BOSTER (California, USA).

Techniques: Infection, Over Expression, Expressing, Knockdown

Journal: International immunopharmacology

Article Title: Sophora subprostrate polysaccharide targets LncRNA MSTRG.5823.1 to suppress PCV2-mediated immunosuppression via TNF/NF-κB signaling.

doi: 10.1016/j.intimp.2024.112701

Figure Lengend Snippet: Fig. 11. SSP promoted activation of the TNF/NF-κB pathway by regulating MSTRG.5823.1/miR-217-x/CXCL2 axis in PCV2- infected RAW264.7 cells (n ¼ 3). Notes: (A) Effect of overexpression of miR-217-x and MSTRG.5823.1 on the expression levels of miR-217-x, MSTRG.5823.1, Rel and TNF-α in SSP treatment group. (B) Effect of overexpression of miR-217-x and MSTRG.5823.1 on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in SSP treatment group. (C-E) Effect of overexpression of miR-217-x and MSTRG.5823.1 on the expression levels of CXCL2, TNF-α, IKB, p-IKB, IKKβ, p-IKKβ, p65and p-p65 proteins in SSP treatment group. (F) Effect of knockdown miR-217-x and MSTRG.5823.1 on the expression levels of miR-217-x, MSTRG.5823.1 and Rel, TNF-α in SSP treatment group. (G) Effect of knockdown miR-217-x and MSTRG.5823.1 on the expression levels of CXCL2, CXCL10, and IL-1β mRNA in SSP treatment group. (H-J) Effect of knockdown miR-217-x and MSTRG.5823.1 on CXCL2, TNF-α, IKB, p-IKB, IKKβ, p-IKKβ, p65 and p-p65 protein expression levels in SSP treatment group. Error bars are represented as means ± SD. Shoulder labels with the same alphabet indicate non-significant differences (p > 0.05), while labels with different alphabets indicate significant differences (p < 0.05).

Article Snippet: The primary antibody against rabbit CXCL2 (pb0900, 1:500 dilution) was obtained from BOSTER (California, USA).

Techniques: Activation Assay, Infection, Over Expression, Expressing, Knockdown

Journal: International immunopharmacology

Article Title: Sophora subprostrate polysaccharide targets LncRNA MSTRG.5823.1 to suppress PCV2-mediated immunosuppression via TNF/NF-κB signaling.

doi: 10.1016/j.intimp.2024.112701

Figure Lengend Snippet: Fig. 12. Effects of SSP on the regulation of PCV2-induced immunosuppression model in mice (n ¼ 12). Notes: (A) Schematic representation of PCV2-induced immunosuppression in vivo model: BALB/c mice were injected intraperitoneally with PCV2 (TCID50 = 10-5/0.1 mL) on the first day of the experiment, and SSP (200 mg/kg•BW) was administered by gavage every day for 7 days. (B-E) secretion levels of CXCL2, CXCL10, TNF-α and IL-1β in thymus tissue homogenate. (F) His- topathological examination of lungs, spleens, livers, and thymus of all three animal groups. [control group, PCV2-infected group, PCV2 + SSP (200 mg/kg•BW)]. scale Bar: 100 µm. (Gray boxes indicate the site of PCV2-induced lesions in tissues. scale Bar: 20 µm.) (G-J) Protein expression levels of TNF-α and CXCL2 in lungs, spleens, livers, and thymuses of all three groups of animals [control group, PCV2-infected group, PCV2 + SSP (200 mg/kg•BW)]. scale Bar: 100 µm. (K) Expression levels of p65 and p-p65 in the thymus from all three animal groups[control group, PCV2-infected group, PCV2 + SSP (200 mg/kg•BW)]. scale Bar: 50 µm. Error bars are represented as means ± SE shoulder labels with the same alphabet indicate non-significant differences (p > 0.05), while labels with different alphabets indicate significant differences (p < 0.05).

Article Snippet: The primary antibody against rabbit CXCL2 (pb0900, 1:500 dilution) was obtained from BOSTER (California, USA).

Techniques: In Vivo, Injection, Control, Infection, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Rosavin Alleviates LPS-Induced Acute Lung Injure by Modulating the TLR-4/NF-κB/MAPK Singnaling Pathways

doi: 10.3390/ijms25031875

Figure Lengend Snippet: Effect of rosavin on p-ERK and p-p38 protein expression in A549 cells. A549 cells were treated with 0, 16, 32, 64 μM rosavin and LPS (1 μg/mL) for 24 h. Data are shown as the mean ± standard deviation ( n = 3); # p < 0.05 vs. control group; * p < 0.05, ** p < 0.01 vs. LPS group.

Article Snippet: ERK (BM4326), MyD88 (BA2321), p38 (BM4142), Bax (A00183), Bcl-2 (BA0412), iNOS (BA0360), COX-2 (BA3708), and CXCL-2 (PB0900) antibody were purchased from Boster Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation, Control

Journal: International Journal of Molecular Sciences

Article Title: Rosavin Alleviates LPS-Induced Acute Lung Injure by Modulating the TLR-4/NF-κB/MAPK Singnaling Pathways

doi: 10.3390/ijms25031875

Figure Lengend Snippet: Effect of rosavin on p-ERK, p-p38, and p-JNK protein expression in lung tissue. Data are shown as the mean ± standard deviation ( n = 3); ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. LPS group.

Article Snippet: ERK (BM4326), MyD88 (BA2321), p38 (BM4142), Bax (A00183), Bcl-2 (BA0412), iNOS (BA0360), COX-2 (BA3708), and CXCL-2 (PB0900) antibody were purchased from Boster Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation, Control

Journal: International Journal of Molecular Sciences

Article Title: Rosavin Alleviates LPS-Induced Acute Lung Injure by Modulating the TLR-4/NF-κB/MAPK Singnaling Pathways

doi: 10.3390/ijms25031875

Figure Lengend Snippet: Effect of rosavin on TLR-4, MyD88, NF-κB p65, and iNOS protein expression in lung tissue. Data are shown as the mean ± standard deviation ( n = 3); ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01 vs. LPS group.

Article Snippet: ERK (BM4326), MyD88 (BA2321), p38 (BM4142), Bax (A00183), Bcl-2 (BA0412), iNOS (BA0360), COX-2 (BA3708), and CXCL-2 (PB0900) antibody were purchased from Boster Biotechnology Co., Ltd., Wuhan, China.

Techniques: Expressing, Standard Deviation, Control